Premium
Preparation of Ready‐to‐use, Stockable and Reconstituted Collagen
Author(s) -
Habermehl Jason,
Skopinska Joanna,
Boccafoschi Francesca,
Sionkowska Alina,
Kaczmarek Halina,
Laroche Gaétan,
Mantovani Diego
Publication year - 2005
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200500102
Subject(s) - stock solution , aqueous solution , ultimate tensile strength , homogeneous , solvent , chemistry , fibroblast , chemical engineering , solubility , materials science , polymer chemistry , composite material , organic chemistry , in vitro , biochemistry , physics , engineering , thermodynamics
Summary: Collagen is a widely used material in biomedical applications. Although processes that prepare collagen and collagen‐based materials that show suitable properties after extraction exist, a ready‐to‐use, easily stockable, with tailored collagen concentration has not yet been developed. Using rat tail tendons, acid soluble collagen solutions were prepared by two different methods. To improve cell viability of pure collagen films, solutions with physiological pH were also prepared by mixing with NaOH solution. Specimens in the form of thin sheets were then fabricated by solvent evaporation. Next, IR spectroscopy, tensile testing techniques as well as human fibroblast cell morphology and cytotoxicity were used to validate the significant variations in the processes. The results demonstrated that, during the synthesis of collagen stock solution, lyophilization and mechanical blending had little effect on the final properties and therefore offers a method for obtaining solutions with a more homogeneous and modifiable collagen concentration and longer storage time. Neutralizing the stock solution with aqueous NaOH prior to solvent evaporation provided films that had lower mechanical properties but significantly improved biological performance.