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Preparation and Characterization of Microwave‐treated Carboxymethyl Chitin and Carboxymethyl Chitosan Films for Potential Use in Wound Care Application
Author(s) -
Wongpanit Panya,
Sanchavanakit Neeracha,
Pavasant Prasit,
Supaphol Pitt,
Tokura Seiichi,
Rujiravanit Ratana
Publication year - 2005
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200500081
Subject(s) - chitosan , chitin , crystallinity , microwave , polymer chemistry , microwave chemistry , nuclear chemistry , materials science , chemistry , autoclave , organic chemistry , composite material , microwave irradiation , physics , quantum mechanics
Abstract Summary: CM‐chitin and CM‐chitosan films were successfully crosslinked by microwave treatment. Crosslinking of the microwave‐treated CM‐chitin films involved mainly the carboxylate and the secondary alcohol groups, while crosslinking of microwave‐treated CM‐chitosan films involved the carboxylate and the amino groups. In addition, the crystallinity of CM‐chitin increased with increasing microwave treatment time, whereas an increase in the crystallinity of the microwave‐treated CM‐chitosan films was not observed. At a similar percentage of weight loss, the crosslinking of either CM‐chitin or CM‐chitosan films by microwave treatment required much less stringent condition when compared with the crosslinking by autoclave treatment. Based on both direct and indirect cytotoxicity assays, the cytotoxicity of the microwave‐treated CM‐chitin films was negative, while that of the microwave‐treated CM‐chitosan films was positive. Human fibroblasts adhered on the surface of microwave‐treated CM‐chitosan films much better than on the surface of microwave‐treated CM‐chitin films.Total amount of protein synthesis of living NHGF cells that were cultured on chitin, microwave‐treated CM‐chitin, chitosan, microwave‐treated CM‐chitosan films.