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Stable Encapsulation of Active Enzyme by Application of Multilayer Nanofilm Coatings to Alginate Microspheres
Author(s) -
Srivastava Rohit,
Brown Jonathan Q.,
Zhu Huiguang,
McShane Michael J.
Publication year - 2005
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200500061
Subject(s) - microsphere , glucose oxidase , chemical engineering , immobilized enzyme , glass microsphere , chemistry , materials science , nanotechnology , biosensor , enzyme , organic chemistry , engineering
Summary: In an effort to improve the stability for long‐term biosensor use, layer‐by‐layer self‐assembly was explored as a potential technique to provide a diffusion barrier to encapsulated glucose oxidase inside alginate microspheres (<5 µm), fabricated using an emulsification technique. The total loss of encapsulated enzyme was reduced to less than 25 and 15% with the application of single PAH/PSS and crosslinked PAH/PAA coatings, respectively, in comparison to at least a 45% loss observed with uncoated and PDDA/PSS‐coated microspheres. Furthermore, it was found that enzyme within PAH/PSS‐ and crosslinked PAH/PAA‐coated spheres retained more than 84 and 60% of initial activity, respectively, after three months, whereas uncoated and PDDA/PSS‐coated microspheres retained less than 20%.Emulsification technique for alginate microspheres.