Premium
Printing Biomacromolecules on a Bovine Serum Albumin Precursor Layer
Author(s) -
Wang Bo,
Feng Jie,
Gao Changyou
Publication year - 2005
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200500037
Subject(s) - microcontact printing , chemistry , bovine serum albumin , horseradish peroxidase , absorbance , substrate (aquarium) , chemical engineering , biophysics , chromatography , biochemistry , biology , oceanography , engineering , enzyme , geology
Summary: Various biomacromolecules including proteins and polysaccharides are printed on a substrate capped with a bovine serum albumin (BSA) precursor layer to create clear co‐patterns of these molecules. Characterizations by confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM) demonstrate the successful production and clear boundaries of the co‐patterns. Rinsing the BSA‐adsorbed substrate and the biomacromolecules‐inked stamp before microcontact printing (µCP) is crucial for the creation of clear and stable co‐patterns. The patterns are mainly stabilized by electrostatic interactions and van der Waals forces. Characterizations by ellipsometry, UV‐Vis and fluorescence spectroscopy reveal that printing by a flat PDMS stamp yields a denser layered structure of proteins with a higher amount than that of adsorbed proteins. By printing, however, a lower enzymatic catalytic activity for horseradish peroxidase (HRP) or binding capability for avidin (both normalized to amount) is determined. A conformational transition from α ‐helix to β ‐sheet of HRP is observed by ATR‐IR. By contrast, a BSA precursor layer can effectively improve the functionality of the printed HRP or avidin and preserve the original conformation of the proteins, although the absolute transferred amount of these proteins is decreased.UV‐Vis absorbance at 405 nm as a function of time showing the activity evolution of HRP on quartz slides (inset) prepared with different methods.