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Highly Branched Stimuli Responsive Poly[( N ‐isopropyl acrylamide)‐ co ‐(1,2‐propandiol‐3‐methacrylate)]s with Protein Binding Functionality
Author(s) -
Carter Steven,
Rimmer Stephen,
Sturdy Alice,
Webb Michelle
Publication year - 2005
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200400218
Subject(s) - methacrylate , isopropyl , acrylamide , chemistry , polymer chemistry , polymer , organic chemistry , copolymer
Summary: Highly branched poly(NIPAM) have been prepared using the technique of reversible addition‐fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain‐ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive “smart” polymers were used to purify a His‐tagged BRCA‐1 protein fragment by affinity precipitation.