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Spectrophotometric Determination of Deacetylation Degree of Chitinous Materials Dissolved in Phosphoric Acid
Author(s) -
Hsiao HsienYi,
Tsai ChihCheng,
Chen Suming,
Hsieh BoChuan,
Chen Richie L.C.
Publication year - 2004
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200400084
Subject(s) - phosphoric acid , acetylation , chemistry , degree (music) , organic chemistry , biochemistry , gene , physics , acoustics
Summary: A simple spectrophotometric method is proposed for determining deacetylation degrees ( DD ) of chitinous materials using phosphoric acid as the UV‐transparent solvent system. Calibrating by the extinction coefficients ( A 210 ) of D ‐glucosamine and N ‐acetyl‐ D ‐glucosamine, DD values (24–88%) were computed numerically. The results correlated well ( R 2 = 0.9805, n = 50) with those obtained by solid‐state 13 C NMR.Comparison of the results obtained by the proposed UV method and solid‐state 13 C NMR.