Biosynthesis and Compositional Regulation of Poly[(3‐hydroxybutyrate)‐ co ‐(3‐hydroxyhexanoate)] in Recombinant  Ralstonia eutropha  Expressing Mutated Polyhydroxyalkanoate Synthase Genes | Zendy

Tsuge Takeharu | Zendy; Saito Yu | Zendy; Kikkawa Yoshihiro | Zendy; Hiraishi Tomohiro | Zendy; Doi Yoshiharu | Zendy

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Biosynthesis and Compositional Regulation of Poly[(3‐hydroxybutyrate)‐ co ‐(3‐hydroxyhexanoate)] in Recombinant Ralstonia eutropha Expressing Mutated Polyhydroxyalkanoate Synthase Genes

Author(s) -

Tsuge Takeharu,

Saito Yu,

Kikkawa Yoshihiro,

Hiraishi Tomohiro,

Doi Yoshiharu

Publication year - 2004

Publication title -

macromolecular bioscience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.924

H-Index - 105

eISSN - 1616-5195

pISSN - 1616-5187

DOI - 10.1002/mabi.200300077

Subject(s) - ralstonia , polyhydroxyalkanoates , cupriavidus necator , gene , chemistry , mutant , biochemistry , operon , biosynthesis , recombinant dna , atp synthase , biology , bacteria , genetics

Summary: A new strategy for bacterial polyhydroxyalkanoate (PHA) production by recombinant Ralstonia eutropha PHB − 4 harboring mutated PHA synthase genes ( phaC Ac ) from Aeromona caviae was investigated. The strain harboring wild‐type phaC Ac gene produced a PHA copolymer consisting of ( R )‐3‐hydroxybutyrate and ( R )‐3‐hydroxyhexanoate [P(3HB‐ co ‐3HHx)] with 3.5 mol‐% of 3HHx fraction from soybean oil. When the mutants of phaC Ac gene were applied to this production system, 3HHx fraction in copolymers was varied in the range of 0–5.1 mol‐%. Thus, the regulation of PHA copolymer compositions has been achieved by the use of mutated PHA synthase genes.Relationship between PHA synthase activity and 3HHx fraction in P(3HB‐ co ‐3HHx).

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