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UV Spectrophotometry: Improvements in the Study of the Degree of Acetylation of Chitosan
Author(s) -
Pedroni Viviana Isabel,
Gschaider María Elena,
Schulz Pablo Carlos
Publication year - 2003
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200300024
Subject(s) - chitosan , chemistry , conductometry , titration , spectrophotometry , glucosamine , chromatography , nuclear chemistry , analytical chemistry (journal) , inorganic chemistry , organic chemistry
A simple and rapid method for determining the degree of N ‐acetylation ( DA ) of chitosan by ultraviolet spectrophotometry using double beam equipment is proposed. D ‐Glucosamine (GlcN) hydrochloride and N ‐acetylglucosamine (GlcNAc) were used as model substances, and chitosan solutions in different solvents were studied. The best results were obtained from 0.1 M HCl solutions, with high reproducibility and in good agreement with those given by conductometric titration, FT‐IR, 1 H NMR and elemental analysis.Correlation between DA obtained by UV and DA obtained by conductometric titration.