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Poly( L ‐lactide)‐Degrading Activity in Various Actinomycetes
Author(s) -
Jarerat Amnat,
Pranamuda Hardaning,
Tokiwa Yutaka
Publication year - 2002
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200290001
Subject(s) - scanning electron microscope , agar , agar plate , 16s ribosomal rna , bacteria , chemistry , biology , materials science , composite material , genetics
The poly( L ‐lactide) (PLA)‐degrading ability of actinomycetes obtained from culture collections was examined by the formation of clear zones on PLA‐emulsified agar plates. Using 41 genera (105 strains) of actinomycetes with phylogenetic affiliations based on 16S rRNA sequences, PLA degraders were found to be limited to members of the family Pseudonocardiaceae and related genera. They included Amycolatopsis , Saccharothrix , Lentzea , Kibdelosporangium , and Streptoalloteichus . A large number of PLA degraders were widely distributed within the genus Saccharothrix . Most strains forming clear zones on PLA‐emulsified agar plates also formed clear zones on silk fibroin agar plates. Saccharothrix species showed an ability to degrade PLA films and assimilate degradation products in liquid cultures. No significant change of the molecular weight and polydispersity ( M w / M n ) of the remaining film fragments was confirmed. After cultivation for two weeks, many irregular holes/pits on the surface of the film due to the colonization of microorganisms were observed by scanning electron microscopy.Scanning electron micrograph of the surface of PLA film: A. orientalis subsp. orientalis IFO 12362 after 14 d.