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Nonspecificity of monoclonal antibody tordji‐22 for the detection of hepatitis C virus in liver transplant recipients with cholestatic hepatitis
Author(s) -
Doughty Alison L.,
Painter Dorothy M.,
McCaughan Geoffrey W.
Publication year - 1999
Publication title -
liver transplantation and surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1074-3022
DOI - 10.1002/lt.500050104
Subject(s) - hepatitis c virus , monoclonal antibody , medicine , liver biopsy , pathology , liver transplantation , hepatitis , staining , hepatitis c , biopsy , hepatocyte , transplantation , population , antigen , antibody , immunohistochemistry , immunology , virus , biology , biochemistry , environmental health , in vitro
Detection of hepatitis C virus (HCV) antigens in liver tissue provides important diagnostic and pathological information. Limited studies have been performed on tissue taken after liver transplantation for HCV. In this study, serial post–liver transplantation biopsy tissue from patients with recurrent HCV was tested, with particular interest in patients showing severe cholestatic hepatitis. HCV‐related antigens were detected using the commercial monoclonal antibody, Tordji‐22. Initial results were promising, showing intense positive staining, especially in areas of hepatocyte ballooning. HCV‐negative donor tissue was consistently negative by staining. However, as a final control for the level of tissue damage, HCV negative posttransplantation biopsy tissue showing hepatocyte ballooning was examined. These tissues also showed positive staining. All attempts to eliminate this nonspecific interaction failed. In conclusion, Tordji‐22 was associated with nonspecificity in this posttransplantation population, and care is warranted when using this monoclonal antibody.