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Detection of circulating donor deoxyribonucleic acid by microsatellite analysis in a liver transplant recipient
Author(s) -
Burt Michael,
Jazwinska Elizabeth,
Lynch Stephen,
Kerlin Paul,
Gill Devinder,
Steadman Charles,
Jonsson Julie,
Strong Russell,
Powell Elizabeth
Publication year - 1996
Publication title -
liver transplantation and surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1074-3022
DOI - 10.1002/lt.500020511
Subject(s) - microsatellite , liver transplantation , rash , pathological , transplantation , allele , typing , liver disease , medicine , biology , pathology , genetics , gene
The diagnosis of graft‐versus‐host disease following liver transplantation may be delayed because the clinical and pathological features are nonspecific. We report the use of microsatellites to support a diagnosis of GVHD in a patient who developed fever and a skin rash 28 days after liver transplantation. The pattern of microsatellite alleles amplified from the peripheral blood on day 51 posttransplant indicated that recipient and donor DNA were present in approximately equal proportions. Microsatellite typing is a simple and rapid method to identify high levels of circulating donor DNA to support a diagnosis of GVHD following liver transplantation. Copyright © 1996 by the American Association for the Study of Liver Diseases.