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Energy status in anoxic rat hepatocytes: Effects of isoflurane, solution composition, and hypothermia
Author(s) -
Howard Ben J.,
Pohorecki Roman,
Becker Gerald L.,
Landers Dennis F.
Publication year - 1995
Publication title -
liver transplantation and surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1074-3022
DOI - 10.1002/lt.500010404
Subject(s) - isoflurane , adenine nucleotide , hypothermia , viaspan , chemistry , anoxic waters , biochemistry , glycolysis , minimum alveolar concentration , transplantation , metabolism , anesthesia , medicine , nucleotide , environmental chemistry , organic chemistry , gene
Abstract Both cold and warm ischemia occur during liver transplantation. Hypothermia and Wisconsin solution preserve adenine nucleotide energy status, which is crucial to hepatic function and viability. The volatile anesthetic isoflurane has been shown to preserve energy status in anoxic isolated hepatocytes in warm Krebs solution. The present study examined isoflurane effects on energy status during incubation also in Wisconsin or Krebs‐plusadenosine solution at 37° or 4°. Hepatocytes were isolated from rat liver after perfusion with Krebs + collagenase. In 25‐mL flasks, 12.5 million cells in 2.5 mL of Krebs, Krebs plus 5 mmol/L adenosine, or Wisconsin solution were incubated under an atmosphere of O 2 /CO 2 or N 2 /CO 2 (19:1) ± isoflurane (3 volumes% = 2ED50), for 30 minutes at 37°C or 4°C. Adenine nucleotides were measured by high‐performance liquid chromatography (HPLC), lactate enzymatically. During warm (37°) anoxia, Wisconsin solution preserved energy status; Krebs plus adenosine did not. Isoflurane further protected energy status in all three solutions. Hypothermia (4°) alone greatly decreased anoxic loss of energy status in all solutions. In Wisconsin solution only, energy status tended to be higher in anoxic than in oxygenated cells and was further enhanced by isoflurane, with corresponding increases in lactate. During 30 minutes of either warm or cold anoxia, isoflurane and Wisconsin solution each helped preserve adenine nucleotide energy status in isolated hepatocytes, at least in part through enhanced glycolysis.