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Phosphatase and tensin homolog–β‐catenin signaling modulates regulatory T cells and inflammatory responses in mouse liver ischemia/reperfusion injury
Author(s) -
Zhu Qiang,
Li Changyong,
Wang Kunpeng,
Yue Shi,
Jiang Longfeng,
Ke Michael,
Busuttil Ronald W.,
KupiecWeglinski Jerzy W.,
Zhang Feng,
Lu Ling,
Ke Bibo
Publication year - 2017
Publication title -
liver transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1527-6465
DOI - 10.1002/lt.24735
Subject(s) - tensin , pten , cancer research , proinflammatory cytokine , signal transduction , microbiology and biotechnology , immunology , medicine , inflammation , biology , pi3k/akt/mtor pathway
The phosphatase and tensin homolog (PTEN) deleted on chromosome 10 plays an important role in regulating T cell activation during inflammatory response. Activation of β‐catenin is crucial for maintaining immune homeostasis. This study investigates the functional roles and molecular mechanisms by which PTEN–β‐catenin signaling promotes regulatory T cell (Treg) induction in a mouse model of liver ischemia/reperfusion injury (IRI). We found that mice with myeloid‐specific phosphatase and tensin homolog knockout (PTEN M‐KO ) exhibited reduced liver damage as evidenced by decreased levels of serum alanine aminotransferase, intrahepatic macrophage trafficking, and proinflammatory mediators compared with the PTEN‐proficient (floxed phosphatase and tensin homolog [PTEN FL/FL ]) controls. Disruption of myeloid PTEN‐activated b‐catenin promoted peroxisome proliferator‐activated receptor gamma (PPARγ)‐mediated Jagged‐1/Notch signaling and induced forkhead box P3 (FOXP3)1 Tregs while inhibiting T helper 17 cells. However, blocking of Notch signaling by inhibiting γ‐secretase reversed myeloid PTEN deficiency‐mediated protection in ischemia/reperfusion–triggered liver inflammation with reduced FOXP3 + and increased retinoid A receptor–related orphan receptor gamma t–mediated interleukin 17A expression in ischemic livers. Moreover, knockdown of β‐catenin or PPARγ in PTEN‐deficient macrophages inhibited Jagged‐1/Notch activation and reduced FOXP3 + Treg induction, leading to increased proinflammatory mediators in macrophage/T cell cocultures. In conclusion, our findings demonstrate that PTEN–β‐catenin signaling is a novel regulator involved in modulating Treg development and provides a potential therapeutic target in liver IRI. Liver Transplantation 23 813–825 2017 AASLD .