Premium
CpG oligodeoxynucleotide triggers the liver inflammatory reaction and abrogates spontaneous tolerance
Author(s) -
Ma LianLi,
Gao Xiudan,
Liu Liping,
Xiang Zhidan,
Blackwell Timothy S.,
Williams Philip,
Chari Ravi S.,
Yin DengPing
Publication year - 2009
Publication title -
liver transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1527-6465
DOI - 10.1002/lt.21771
Subject(s) - medicine , liver transplantation , cpg site , cpg oligodeoxynucleotide , transplantation , genetics , gene , dna methylation , gene expression , biology
Liver allografts are spontaneously accepted in the liver transplantation mouse model; however, the basis for this tolerance and the conditions that abrogate spontaneous tolerance to liver allografts are incompletely understood. We examined the role of CpG oligodeoxynucleotide (ODN) in triggering the liver inflammatory reaction and allograft rejection. Bioluminescence imaging quantified the activation of nuclear transcriptional factor κB (NF‐κB) at different time points post‐transplantation. Intrahepatic lymphocyte subsets were analyzed by immunofluorescence assay and flow cytometry. The results showed that liver allografts survived for more than 100 days without a requirement for any immunosuppressive therapy. Donor‐matched cardiac allografts were permanently accepted, whereas third‐party cardiac grafts were rejected with delayed kinetics; this confirmed donor‐specific tolerance. NF‐κB activation in the liver allografts was transiently increased on day 1 and diminished by day 4; in comparison, it was elevated up to 10 days post‐transplantation in the cardiac allografts. When CpG ODN was administered at a high dose (50 μg per mouse × 1) to the recipients on day 7 post‐transplantation, it induced an acute liver inflammatory reaction with elevated NF‐κB activation in both allogeneic and syngeneic liver grafts. Multiple doses of CpG ODN (10 μg per mouse × 3) elicited acute rejection of the liver allografts with significant T cell infiltration in the liver allografts, reduced T regulatory cells, and enhanced interferon gamma–producing cells in the intrahepatic infiltrating lymphocytes. These data demonstrate that CpG ODN initiates an inflammatory reaction and abrogates spontaneous tolerance in the liver transplantation mouse model. Liver Transpl 15:915–923, 2009. © 2009 AASLD.