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Tissue factor‐dependent procoagulant activity of isolated human hepatocytes: Relevance to liver cell transplantation
Author(s) -
Stéphenne Xavier,
Vosters Olivier,
Najimi Mustapha,
Beuneu Claire,
Ngoc Dung Khuu,
Wijns Walter,
Goldman Michel,
Sokal Etienne M.
Publication year - 2007
Publication title -
liver transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1527-6465
DOI - 10.1002/lt.21128
Subject(s) - tissue factor , hepatocyte , transplantation , medicine , thromboplastin , liver transplantation , coagulation , in vitro , cell , flow cytometry , immunology , microbiology and biotechnology , andrology , cancer research , biochemistry , chemistry , biology
Liver cell transplantation (LCT) aims to correct inborn liver function defects by infusing metabolically active cells into the diseased liver. Further improvement in LCT might depend on the prevention of early loss of transplanted cells. As tissue factor (TF)‐dependent activation of coagulation was found to contribute to a low rate of beta cell engraftment in islet transplantation, we investigated the potential procoagulant activity (PCA) of hepatocyte preparations. TF expression on hepatocyte preparations was assessed by flow cytometry, reverse‐transcription polymerase chain reaction and immunofluorescence. PCA depending on TF was evaluated in human plasma and in whole blood systems. Coagulation parameters were followed by routine techniques in a LCT recipient Crigler‐Najjar patient. We determined that hepatocytes express soluble and membrane‐bound forms of TF. We showed that hepatocytes exert a TF‐dependent PCA. In parallel, delayed increase in D‐dimer levels was observed following the hepatocyte infusions in the Crigler‐Najjar patient. Furthermore, in vitro experiments demonstrated that TF‐dependent PCA of hepatocytes is inhibited by N‐acetyl‐L‐cysteine. In conclusion, hepatocytes exert TF‐dependent PCA, which may contribute to early loss of infused cells. Addition of N‐acetyl‐L‐cysteine to the suspensions of hepatocytes might be beneficial in LCT by inhibiting activation of coagulation. Liver Transpl 13:599–606, 2007. © 2007 AASLD.