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Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis
Author(s) -
NavarroSabaté Àurea,
Peralta Carmen,
Calvo Maria Nieves,
Manzano Anna,
MassipSalcedo Marta,
RosellóCatafau Joan,
Bartrons Ramon
Publication year - 2006
Publication title -
liver transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1527-6465
DOI - 10.1002/lt.20863
Subject(s) - ischemic preconditioning , ischemia , medicine , liver transplantation , gene expression , gene , microarray analysis techniques , microarray , warm ischemia , pharmacology , reperfusion injury , transplantation , biology , biochemistry
Abstract Hepatic ischemia‐reperfusion injury associated with liver transplantation is an as yet unresolved problem in clinical practice. Preconditioning protects the liver against the deleterious effects of ischemia, although the mechanism underlying this preconditioning is still unclear. To profile gene expression patterns involved in hepatic ischemic preconditioning, we analyzed the changes in gene expression in rat livers by DNA microarray analysis. Approximately 116 genes were found to have altered gene expression after 8 hours of cold ischemia. Moreover, the expression of 218 genes was modified by classic preconditioning followed by the same ischemia process. Given the importance of the effects of ischemic preconditioning (IP) in minimizing the liver damage induced by sustained ischemia before reperfusion, this study analyzed the putative genes involved in the beneficial role of IP in liver grafts undergoing cold ischemia before its implantation in the recipient (IP+I). Great differences were found in the gene expression pattern of ischemic preconditioning + long cold ischemia (IP+I) group when compared with the long cold ischemia alone condition (I), which could explain the protective regulatory mechanisms that take place after preconditioning. Twenty‐six genes that were downregulated in cold ischemia were found upregulated after preconditioning preceding a long cold ischemia period. These would be genes activated or maintained by preconditioning. Heat shock protein genes and 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase are among the most markedly induced transcripts. Liver Transpl. 12:1615–1625, 2006. © 2006 AASLD.