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Activation pattern of mitogen‐activated protein kinases in early phase of different size liver isografts in rats
Author(s) -
Liang Tingbo,
Xu Shiguo,
Yu Jun,
Shen Kezhen,
Li Donglin,
Zheng Shusen
Publication year - 2005
Publication title -
liver transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1527-6465
DOI - 10.1002/lt.20518
Subject(s) - medicine , kinase , liver transplantation , mitogen activated protein kinase , protein kinase a , microbiology and biotechnology , transplantation , biology
Mitogen‐activated protein kinases (MAPK) play a pivotal role in ischemia reperfusion injuries of heart and liver, but the activation pattern of MAPKs in the early phase of different size liver isografts remains unclear. The experiment is designed to investigate the activation pattern and role of MAPKs in isografts of the rat with different size liver transplantation. The animal models of different size graft liver transplantation (whole graft, 50% size, or 30% size, respectively) were established and the sham operation group served as a control. The recipients were sacrificed at 0.5‐, 2‐, 6‐, and 24‐hour time points after transplantation to harvest the graft specimens and blood samples. The serum aspartate amino transferase (AST), alanine amino transferase (ALT) and tumor necrosis factor‐α (TNF‐α) levels, and histological findings were evaluated. The expressions of the total and phosphorylated p46/p54 JNKs, p38 MAPK, and p42/p44 ERKs were detected by Western blot. The serum ALT and AST levels increased significantly at the 0.5‐hour time point and maintained high with the peak levels at the 6‐hour time point after liver transplantation. The different sizes of liver isografts did not change the expressions of total p46/p54JNKs, p38MAPK, and p42/p44 ERKs. While the expressions of phosphorylated p46/p54JNKs, p38 MAPK, and p42/p44 ERKs were either negative or mildly up‐regulated in the sham operation group, they were significantly activated in the transplanted liver at the 0.5‐hour time point, especially in the 30% size liver transplantation group. In conclusion, the activation of three MAPKs in liver isografts correlates with graft size and the JNK and p38 MAPK are responsible for the graft injury while the ERK signal pathway maybe participate in the regulation of cell growth and differentiation after small‐for‐size liver transplantation. (Liver Transpl 2005;11:1527–1532.)