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Involvement of Aquaporins in the Intense Pulsed Light‐Enhanced Wound Healing in Diabetic Rats
Author(s) -
Huang PoWei,
Jeng SengFeng,
Liu ChiMing,
Chen ChienChung,
Chang LiRen,
Shih HsiangShun,
Chen HsingFu,
Yang ChihHui,
Chen JianAn,
Feng GuanMing
Publication year - 2021
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.23303
Subject(s) - wound healing , downregulation and upregulation , streptozotocin , diabetes mellitus , aquaporin , dorsum , medicine , endocrinology , chemistry , surgery , anatomy , physiology , biochemistry , gene
Background and Objectives We previously demonstrated that intense pulsed light (IPL) irradiation prior to wounding improved the wound healing in rats with diabetes mellitus (DM). Also, we found that IPL upregulated the expression of aquaporin 3 (AQP3), a protein that is crucial for wound healing, in normal rats. This present study aimed to examine the involvement of AQPs in the IPL‐enhanced wound healing in diabetic rats. Study Design/Materials and Methods Streptozotocin was used to induce diabetes in Sprague–Dawley rats. Animals were divided into four groups: normal group, DM only group, DM rats with IPL treatment 2 weeks before wounding (DM + IPL‐Pre group), and DM rats with concurrent IPL irradiation and wounding (DM + IPL‐Con group). Wounds were created on the dorsal skin of rats. The expressions of AQP1, 3, 4, 7, and 9 in the pre‐injured skin, periwound, and wound were determined. Results Among all the AQPs analyzed, only the expressions of AQP3 and AQP7 were significantly altered. Unirradiated diabetic rats showed much higher expression level of AQP3 in the regenerating skin compared with normal rats. IPL pretreatment, but not concurrent treatment, attenuated the expression toward the level detected in the normal wounds. In contrast, a lower expression level of AQP7 was noted in the regenerating skin of DM only rats and IPL pretreatment upregulated the expression to a level similar to that in the normal rats. Conclusion The beneficial effect of IPL pretreatment on the wound healing in diabetic rats might involve a mechanism by which the expression of AQPs is regulated. Lasers Surg. Med. © 2020 Wiley Periodicals LLC