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Hydrogen‐Peroxide‐Responsive Protein Biomimetic Nanoparticles for Photothermal‐Photodynamic Combination Therapy of Melanoma
Author(s) -
Wen Long,
Hyoju Riza,
Wang Peiru,
Shi Lei,
Li Chunxiao,
Li Meng,
Wang Xiuli
Publication year - 2021
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.23292
Subject(s) - indocyanine green , photothermal therapy , photodynamic therapy , in vivo , chemistry , singlet oxygen , hydrogen peroxide , melanoma , biocompatibility , bovine serum albumin , reactive oxygen species , cancer research , materials science , pathology , medicine , nanotechnology , oxygen , biochemistry , biology , organic chemistry , microbiology and biotechnology
Background and Objectives Recently, there has been a rapid increase in the incidences of melanoma, which represents a serious threat to human health. Generally, tumor‐microenvironment‐responsive nanoparticle‐based photothermal‐photodynamic combination therapy (PTT‐PDT) is characterized by intratumoral response and tumor targeting. In this study, we designed and synthesized hydrogen‐peroxide‐responsive protein biomimetic nanoparticles (MnO 2 ‐ICG@BSA) for the treatment of melanoma. Study Design/Materials and Methods Briefly, MnO 2 ‐ICG@BSA was prepared using a mild protein synthesis method by loading indocyanine green (ICG) into a bovine serum albumin–manganese dioxide complex (MnO 2 @BSA); next, its characteristics were determined. In addition, in vitro biocompatibility and antitumor efficacy were assessed using the classic cell counting kit‐8 assay. Moreover, in vivo high‐frequency ultrasound and thermal imaging were used to evaluate the oxygen‐production capacity and photothermal conversion effect of MnO 2 ‐ICG@BSA at the tumor site, and Singlet Oxygen Sensor Green (SOSG) was used to measure singlet oxygen levels in the tumor. The antitumor efficacy was assessed based on relative tumor size, bodyweight, survival curves, and hematoxylin and eosin staining. Results The results showed that MnO 2 ‐ICG@BSA has a high photothermal conversion efficiency, a strong singlet oxygen‐generation ability, and high photothermal stability. In addition, in vitro PTT‐PDT experiments showed that MnO 2 ‐ICG@BSA has a significant inhibitory effect on the proliferation of B16F10 melanoma cells. Meanwhile, in vivo experiments showed that MnO 2 ‐ICG@BSA has a significant inhibitory effect on melanoma in mice. Preliminary toxicity studies indicated that MnO 2 ‐ICG@BSA exhibits low toxicity. Conclusion From the results, we can conclude that MnO 2 ‐ICG@BSA could be used in PTT‐PDT to treat melanoma, making it a good candidate material for PTT‐PDT. Lasers Surg. Med. © 2020 Wiley Periodicals LLC