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Prevention of Local Tumor Recurrence After Surgery by Thermosensitive Gel‐Based Chemophotothermal Therapy in Mice
Author(s) -
Fan Yanyan,
Yu Dujuan,
Li Duan,
Wang Xue
Publication year - 2020
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.23206
Subject(s) - medicine , poloxamer 407 , doxorubicin , poloxamer , surgery , photodynamic therapy , cytotoxicity , viability assay , urology , in vitro , chemotherapy , chemistry , polymer , biochemistry , organic chemistry , copolymer
Background and Objectives Local recurrence of cancer after surgery has long been a tough problem. In the present study, thermosensitive gel‐based chemophotothermal therapy was applied to prevent the recurrence of liver cancer after surgery. Study Design/Materials and Methods Mesoporous silica nanoparticles (MSNs) were used as first‐level carrier to co‐load doxorubicin (DOX) and ICG. Then, the drug‐loaded MSNs (D‐I@MSN) were incorporated into poloxamer gel. A mimic model of liver cancer recurrence after surgery was prepared by subcutaneously injecting H22 cells into the armpit of mice. Then the two‐level composite gel (D‐I@MSN/gel) was also subcutaneously injected at the same site before the formation of tumor, followed by 808 nm laser irradiation. Results The loading efficiency and entrapment efficiency of DOX were as high as 8.85% and 96.9%, and that of ICG were 9.24% and 99.3%, respectively. The results of in vitro cytotoxicity showed that cell viability in D‐I@MSN+Laser group was only 5.8% after being irradiated by 808 nm laser for 5 minutes (0.5 W/cm 2 ). In animal studies, tumor formation (tumor recurrence) was greatly inhibited in D‐I@MSN+Laser group. Conclusions The thermosensitive gel‐based chemophotothermal therapy showed excellent safety and efficacy when applied in the prevention of mimic local tumor replase after surgery in mice, presenting its great potential clinically. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.