Premium
Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair
Author(s) -
Andreo Lucas,
MesquitaFerrari Raquel A.,
Ribeiro Beatriz G.,
Benitte Adriana,
de Fátima Nogueira Tatiane,
França Cristiane M.,
Silva Daniela de Fátima Teixeira da,
Bussadori Sandra K.,
Fernandes Kristianne P.S.,
Corrêa Fernanda I.,
Corrêa João C.F.
Publication year - 2018
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22798
Subject(s) - transplantation , c2c12 , medicine , tibialis anterior muscle , skeletal muscle , myocyte , pathology , balanced salt solution , wound healing , surgery , chemistry , anatomy , myogenesis , organic chemistry
Objective The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. Materials and Methods Sixty‐five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages. Results No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days. Conclusion Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018. © 2018 Wiley Periodicals, Inc.