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Combination of platelet rich plasma in fractional carbon dioxide laser treatment increased clinical efficacy of for acne scar by enhancement of collagen production and modulation of laser‐induced inflammation
Author(s) -
Min Seonguk,
Yoon Ji Young,
Park Seon Yong,
Moon Jungyoon,
Kwon Hyuck Hoon,
Suh Dae Hun
Publication year - 2018
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22776
Subject(s) - platelet rich plasma , erythema , scars , medicine , acne scars , fibroblast , wound healing , acne , inflammation , biopsy , adverse effect , dermatology , pathology , surgery , chemistry , platelet , in vitro , biochemistry
Background Platelet‐rich plasma (PRP) which contains large amounts of growth factors has been tried to enhance therapeutic efficacy of laser treatment for acne scar with unknown underlying mechanism. Objectives The present study was conducted to investigate the molecular mechanism of increased clinical efficacy of PRP when combined with fractional laser treatment for treating acne scars. Methods Subjects with mild to moderate acne scars were treated with two sessions of fractional CO 2 laser therapy given with and without co‐administration of PRP. Skin biopsy specimens were obtained at baseline, 1, 3, 7, and 28 days for investigation of molecular profiles associated with skin changes produced by laser plus PRP treatment. Results The PRP treatment increased clinical efficacy with decreased severity of adverse effects such as erythema, swelling and oozing. Productions of TGFβ1 and TGFβ3 proteins were more highly elevated on the PRP‐treated side of the face compared to the control side at day 28. Furthermore, PRP‐treated side showed significant increase of c‐myc, TIMP, and HGF expression. Experimental fibroblast culture model was also used. PRP administration after laser irradiation increased expressions of p‐Akt, TGFβ1, TGFβ3, β‐catenin, collagen 1, and collagen 3 in both dose‐dependent and time dependent manners in fibroblast. Moreover, we acquired clinical and histological data through randomized control clinical trial. Conclusion Taken together with human study results combined with the data from cell experiments we suggest that PRP treatment increased fibrogenetic molecules induced by fractional CO 2 laser, which have association with clinical effect. Lasers Surg. Med. 50:302–310, 2018. © 2017 Wiley Periodicals, Inc.