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Treatment of keloid scars with a 1210‐nm diode laser in an animal model
Author(s) -
Philandrianos Cécile,
Bertrand Baptiste,
AndracMeyer Lucile,
Magalon Guy,
Casanova Dominique,
Kerfant Nathalie,
Mordon Serge
Publication year - 2015
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22428
Subject(s) - keloid , scars , medicine , laser , resection , wound healing , surgery , animal model , optics , physics
Background and Objective A temperature increase can improve wound healing by activation of heat shock protein 70 and stimulation of fibroblasts. Since keloids are a dysfunction of collagen fiber synthesis and organization, this study aimed to evaluate if a 1,210 nm diode laser could have effects in a new animal model of keloid scars. Study Design/Materials and Methods A total of 39 nude mice were used for this study. Phototypes IV and V human keloids were grafted into their backs and after 1 month of healing, the mice were divided into four groups: Control, Laser, Resection, Resection/Laser. In the Laser group, the keloids were treated with a 1,210‐nm diode‐laser with the following parameters: 4 W; 10 seconds; fluence: 51 J/cm 2 ; spot: 18.9 × 3.7 mm 2 . In the Resection group, surgical intra‐lesional excision was performed. In the Resection/Laser group, keloids were treated with the 1,210‐nm laser‐diode after surgical intra‐lesional excision. Temperature measurements were made during the laser treatment. Clinical examination and histological study were performed on the day of treatment and 1 month, 2 months, and 3 months later. Results Mean temperature measurement was of 44.8°C (42–48°) in the Laser groups. No healing complications or keloid proliferation was observed in any group. Keloid histologic characters were confirmed in all grafts. No histologic particularity was observed in the laser groups in comparison with the Control and Resection groups. Conclusion First, this keloid animal model appears to be adapted for laser study. Secondly, the 1,210‐nm diode laser does not induce keloid thermal damage in vivo . Further studies with different 1,210‐nm laser diode parameters should be performed in order to observe significant effects on keloids. Lasers Surg. Med. 47:798–806, 2015. © 2015 Wiley Periodicals, Inc.