Premium
Detection of plaque structure and composition using OCT combined with two‐photon luminescence (TPL) imaging
Author(s) -
Wang Tianyi,
McElroy Austin,
Halaney David,
Vela Deborah,
Fung Edmund,
Hossain Shafat,
Phipps Jennifer,
Wang Bingqing,
Yin Biwei,
Feldman Marc D.,
Milner Thomas E.
Publication year - 2015
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22366
Subject(s) - optical coherence tomography , biomedical engineering , fibrous cap , materials science , pathology , intravascular ultrasound , ex vivo , context (archaeology) , vulnerable plaque , elastin , chemistry , medicine , radiology , in vitro , biology , paleontology , biochemistry
Background and Objectives Atherosclerosis and plaque rupture leads to myocardial infarction and stroke. A novel hybrid optical coherence tomography (OCT) and two‐photon luminescence (TPL) fiber‐based imaging system was developed to characterize tissue constituents in the context of plaque morphology. Study Design/Materials and Methods Ex vivo coronary arteries (34 regions of interest) from three human hearts with atherosclerotic plaques were examined by OCT–TPL imaging. Histological sections (4 μm in thickness) were stained with Oil Red O for lipid, Von Kossa for calcium, and Verhoeff–Masson Tri‐Elastic for collagen/elastin fibers and compared with imaging results. Results Biochemical components in plaques including lipid, oxidized‐LDL, and calcium, as well as a non‐tissue component (metal) are distinguished by multi‐channel TPL images with statistical significance ( P < 0.001). TPL imaging provides complementary optical contrast to OCT (two‐photon absorption/emission vs scattering). Merged OCT–TPL images demonstrate the distribution of lipid deposits in registration with detailed plaque surface profile. Conclusions Results suggest that multi‐channel TPL imaging can effectively identify lipid sub‐types and different plaque components. Furthermore, fiber‐based hybrid OCT–TPL imaging simultaneously detects plaque structure and composition, improving the efficacy of vulnerable plaque detection and characterization. Lasers Surg. Med. 47:485–494, 2015. © 2015 Wiley Periodicals, Inc.