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DNA damage in blood cells exposed to low‐level lasers
Author(s) -
Sergio Luiz Philippe da Silva,
Silva Ana Paula Almeida da,
Amorim Philipi Freitas,
Campos Vera Maria Araújo,
Magalhães Luis Alexandre Gonçalves,
de Paoli Flavia,
de Souza da Fonseca Adenilson
Publication year - 2015
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22344
Subject(s) - comet assay , dna damage , laser , dna repair , dna , chemistry , medicine , optics , biochemistry , physics
Background and Objective In regenerative medicine, there are increasing applications of low‐level lasers in therapeutic protocols for treatment of diseases in soft and in bone tissues. However, there are doubts about effects on DNA, and an adequate dosimetry could improve the safety of clinical applications of these lasers. This work aimed to evaluate DNA damage in peripheral blood cells of Wistar rats induced by low‐level red and infrared lasers at different fluences, powers, and emission modes according to therapeutic protocols. Material and Methods Peripheral blood samples were exposed to lasers and DNA damage was accessed by comet assay. In other experiments, DNA damage was accessed in blood cells by modified comet assay using formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III enzymes. Results Data show that exposure to low‐level red and infrared lasers induce DNA damage depending on fluence, power and emission mode, which are targeted by Fpg and endonuclease III. Conclusion Oxidative DNA damage should be considered for therapeutic efficacy and patient safety in clinical applications based on low‐level red and infrared lasers. Lasers Surg. Med. 47:361–368, 2015. © 2015 Wiley Periodicals, Inc.