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The effect of 595 nm pulsed dye laser on connective tissue growth factor (CTGF) expression in cultured keloid fibroblasts
Author(s) -
Zhu Rongyi,
Yue Baishuang,
Yang Qiaorong,
Ma Ying,
Huang Guiqin,
Guan Ming,
Avram Mathew M.,
Lu Zhong
Publication year - 2015
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22334
Subject(s) - ctgf , keloid , connective tissue , growth factor , pathology , transforming growth factor , chemistry , medicine , cancer research , materials science , receptor
Objective To investigate the effect of pulsed dye laser (PDL 595 nm) on the proliferation and expression of connective tissue growth factor (CTGF) in cultured keloid fibroblasts. Materials and Methods Cultured keloid fibroblasts were exposed to pulsed dye laser irradiation at fluences of 6, 8, and 10 J/cm 2 , with pulse durations of 1.5, 3, and 10 ms. The viability of keloid fibroblasts was measured with CCK‐8 at 72, 24, and 12 hours prior to irradiation. Subsequently, viability was measured at 12, 24, and 72 hours post‐irradiation. Additionally, the fibroblast cell cycle and apoptosis rate were measured by flow cytometry. Finally, keloid fibroblasts underwent real‐time polymerase chain reaction (PCR) and Western blot to investigate the CTGF mRNA and protein expression after PDL irradiation. The untreated cultured keloid fibroblasts served as controls. Results The proliferation of keloid fibroblasts was significantly inhibited after PDL irradiation. Both CTGF mRNA and protein expression were significantly down‐regulated in 1.5, 3, and 10 ms pulse duration groups, in a dose dependent manner ( P < 0.05). However, there was no statistically significant difference between groups of different pulse duration in 6, 8, and 10 J/cm 2 fluence ranges ( P > 0.05). Conclusions Within certain fluence ranges, pulsed dye laser can effectively suppress the growth of keloids and significantly down‐regulate CTGF mRNA and CTGF expression. Lasers Surg. Med. 47:203–209, 2015. © 2015 Wiley Periodicals, Inc.