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Ex vivo laser thermoplasty of whole costal cartilages
Author(s) -
Sviridov Alexander P.,
Zakharkina Olga L.,
Ignatieva Natalia Yu.,
Vorobieva Natalia N.,
Bagratashvili Nodar V.,
Plyakin Vladimir A.,
Kulik Innokentii O.,
Sarukhanyan Oganes O.,
Minaev Vladimir P.,
Lunin Valerii V.,
Bagratashvili Victor N.
Publication year - 2014
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22233
Subject(s) - laser , ex vivo , costal cartilage , cartilage , biomedical engineering , materials science , fiber laser , irradiation , wavelength , medicine , in vivo , anatomy , nuclear medicine , optics , optoelectronics , biology , physics , microbiology and biotechnology , nuclear physics
Background and Objective To examine the possibilities of laser thermoplasty of whole costal cartilages for correction the human congenital chest wall deformities. Study Design/Materials and Methods Ex vivo the samples of porcine costal cartilages were heated with lasers of differing wavelengths, including a 0.97‐μm diode laser, a 1.56‐μm erbium‐doped quartz fiber laser, and a 1.68‐μm fiber Raman laser. The dynamics of the temperature fields and the degradation of collagen in the laser‐affected regions of samples were determined by using, respectively, thermometry, trypsin treatment, and light microscopy . Ex vivo the whole mechanically deformed costal cartilages of pigs were treated by laser radiation with wavelength 1.68‐μm. The changes of cartilage shape were recorded at certain intervals over a 24‐hour period by photographing them in a fixed position with a digital camera. Results Treatment of costal cartilage samples from 5 to 11 mm in thickness by laser radiation with 0.97, 1.56, and 1.68 μm wavelengths showed that the 1.68‐μm radiation could produce the necessary nonuniform bulk heating of the exposed sample. The altered shape of costal cartilage proved to remain stable after treatment when the laser irradiation settings used provided for the heating of a broad region within the tissue to temperatures about 80°C. Conclusion This study demonstrates the possibilities of laser thermoplasty of whole costal cartilages for treatment of human congenital chest wall deformities. The development of novel approaches based on laser cartilage engineering techniques will enable to treat the human congenital chest wall deformities. Lasers Surg. Med. 46:302–309, 2014. © 2014 Wiley Periodicals, Inc.