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Recovery of intrinsic fluorescence from single‐point interstitial measurements for quantification of doxorubicin concentration
Author(s) -
Baran Timothy M.,
Foster Thomas H.
Publication year - 2013
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22166
Subject(s) - fluorophore , fluorescence , materials science , context (archaeology) , doxorubicin , analytical chemistry (journal) , scattering , fluorescence spectroscopy , chemistry , optics , chromatography , physics , medicine , surgery , paleontology , chemotherapy , biology
Background and Objective We developed a method for the recovery of intrinsic fluorescence from single‐point measurements in highly scattering and absorbing samples without a priori knowledge of the sample optical properties. The goal of the study was to demonstrate accurate recovery of fluorophore concentration in samples with widely varying background optical properties, while simultaneously recovering the optical properties. Materials and Methods Tissue‐simulating phantoms containing doxorubicin, MnTPPS, and Intralipid‐20% were created, and fluorescence measurements were performed using a single isotropic probe. The resulting spectra were analyzed using a forward‐adjoint fluorescence model in order to recover the fluorophore concentration and background optical properties. Results We demonstrated recovery of doxorubicin concentration with a mean error of 11.8%. The concentration of the background absorber was recovered with an average error of 23.2% and the scattering spectrum was recovered with a mean error of 19.8%. Conclusion This method will allow for the determination of local concentrations of fluorescent drugs, such as doxorubicin, from minimally invasive fluorescence measurements. This is particularly interesting in the context of transarterial chemoembolization (TACE) treatment of liver cancer. Lasers Surg. Med. 45:542–550, 2013. © 2013 Wiley Periodicals, Inc.