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Minimally Invasive Bipolar Fractional Radiofrequency Treatment Upregulates Anti‐ S enescence Pathways
Author(s) -
Rangarajan Sachin,
Trivedi Apexa,
Ubeid Anan Abu,
Hantash Basil M.
Publication year - 2013
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22135
Subject(s) - senescence , sirtuin , sirtuin 1 , foxo3 , apoptosis , gene expression , medicine , signal transduction , resveratrol , cancer research , gene , biology , pharmacology , downregulation and upregulation , microbiology and biotechnology , acetylation , biochemistry
Background The sirtuin gene family has been implicated in various anti‐senescence pathways. Its connection, if any, with the skin wound healing response has yet to be elucidated. Objective The goal of our study was to better understand the effects of FRF treatment on the sirtuin anti‐senescence pathway in skin. Methods Human abdominal skin was treated with FRF, and then harvested at 0, 2, 14, and 28 days post‐treatment to assess for temporal changes in gene expression levels. Results Decreased levels of SIRT1, 3, 5, and 7 were observed immediately post‐FRF treatment. By Day 2, SIRT1, 6, and 7 expressions increased 50–100%. SIRT6 and 7 expression continued to increase through Day 28. Expression levels of apoptosis genes FoxO3 and p53 decreased, while Bax levels increased by Day 28. Conclusions Our results raise the possibility that sirtuin activity may be used as an accurate corollary to clinical improvement in skin quality. Lasers Surg. Med. 9999:XX–XX, 2013. © 2013 Wiley Periodicals, Inc.