Premium
Validation of an ex vivo human cervical tissue model for optical imaging studies
Author(s) -
Gallwas Julia,
Mortensen Uwe,
Gaschler Rebecca,
Ochsenkuehn Robert,
Stepp Herbert,
Friese Klaus,
Dannecker Christian
Publication year - 2012
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.22001
Subject(s) - ex vivo , optical coherence tomography , medicine , histology , intensity (physics) , in vivo , nuclear medicine , biomedical engineering , pathology , radiology , biology , optics , physics , microbiology and biotechnology
Objective To establish and validate an ex vivo human cervical tissue model for optical coherence tomography (OCT) studies with special emphasis on investigating time dependent structural changes of the epithelium. Methods Four hundred OCT images were taken from 80 unsuspicious and suspicious areas of 18 fresh conisation specimens immediately after resection (0 hour) and 1, 2, 3, and 4 hours, referred to as waiting times in the following, postoperatively and compared to the corresponding histology. For each 2D‐OCT‐image, a 1D‐intensity profile was generated. The profiles were analyzed with respect to systematic differences which may result from different waiting times, employing discriminant analysis (DA). Results The different waiting times do not appear to imply significant differences among the corresponding OCT‐images as represented by the intensity profiles. Conclusion We conclude that the proposed cervical tissue model appears to be applicable for ex vivo OCT studies. Lasers Surg. Med. 44:245–248, 2012. © 2012 Wiley Periodicals, Inc.