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Assessment of pulpal vitality using laser speckle imaging
Author(s) -
Stoianovici Charles,
WilderSmith Petra,
Choi Bernard
Publication year - 2011
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.21090
Subject(s) - transillumination , speckle pattern , biomedical engineering , materials science , dentistry , laser , perfusion , blood flow , medicine , optics , pathology , radiology , physics
Abstract Background and Objective The pulpal chamber of each tooth contains the vasculature necessary to maintain a viable tooth. A critical need exists to develop an objective, repeatable method to assess pulpal viability. We hypothesized that the existence of blood perfusion within the pulp can be determined with analysis of laser speckle imaging (LSI) patterns generated by transillumination of the tooth. Study Design/Materials and Methods We used nine extracted human cuspids and incisors. A Tygon tube was inserted into a channel created within each tooth and Intralipid pumped through the tube in a controlled manner with a syringe infusion pump. We evaluated the feasibility of LSI for flow assessment using both transillumination and epiillumination imaging configurations. With the transillumination geometry, we also assessed the effect of the angle of incidence of the probe laser light on the speckle flow index (SFI) values extracted from the collected speckle images. Results Transillumination LSI, and not epiillumination LSI, enables differentiation between the absence and presence of perfusion in an in vitro tooth model. SFI values are insensitive to the relative angle of incidence of the laser light, over a wide range of angles. Conclusions Our preliminary in vitro data suggest that transillumination LSI is a promising method to identify the presence of blood flow in the pulpal chamber. Future in vivo evaluation is warranted. Lasers Surg. Med. © 2011 Wiley‐Liss, Inc.