Premium
Effects of 635nm light‐emitting diode irradiation on angiogenesis in CoCl 2 ‐exposed HUVECs
Author(s) -
Lim Won Bong,
Kim Ji Sun,
Ko Young Jong,
Kwon HyukIl,
Kim Sang Woo,
Min Heung Kee,
Kim Oksu,
Choi Hong Ran,
Kim Ok Joon
Publication year - 2011
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.21038
Subject(s) - angiogenesis , mapk/erk pathway , umbilical vein , oxidative stress , vascular endothelial growth factor , reactive oxygen species , viability assay , chemistry , intracellular , hypoxia (environmental) , wound healing , vascular endothelial growth factor a , microbiology and biotechnology , cancer research , kinase , medicine , biology , cell , in vitro , immunology , biochemistry , vegf receptors , oxygen , organic chemistry
Background and Objectives It is recognized that hypoxic/ischemic conditions leading to production of reactive oxygen species (ROS) are an important mediator of angiogenesis in the wound‐healing process. Recently, low level light irradiation at 635 nm, which is used in many clinical fields, was found to decrease intracellular ROS levels, and consequently alleviate oxidative stress. The purpose of the present study was to investigate the effects of 635 nm light‐emitting diode (LED) irradiation on angiogenesis in human umbilical vein endothelial cells, in an in vitro CoCl 2 ‐induced severe hypoxia model. Study Design/Materials and Methods The effects were assessed on cell viability, tube formation, hypoxia‐inducible factor‐1, vascular endothelial growth factor (VEGF), VEGF‐1 and ‐2 protein expression, mitogen‐activated protein kinase (MAPK) phosphorylation, and ROS dissociation. Results The results showed that, under hypoxic/ischemic conditions, irradiation with 635 leads to reduced production and increased scavenging of intracellular ROS, which results in alleviation of VEGFR‐1 suppression, enhanced VEGF expression and ERK MAPK activation, and subsequent acceleration of angiogenesis with improved cell viability and tube formation. Conclusion Taken together, irradiation with 635 nm was shown to reduce intracellular ROS production, which results in increased angiogenesis. Thus, we suggest that irradiation with 635 nm accelerate angiogenesis under hypoxic/ischemic conditions, and may prove to be a useful alternative tool in wound healing. Lasers Surg. Med. 43:344–352, 2011. © 2011 Wiley‐Liss, Inc.