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Increase in matrix metalloproteinase‐2 level in the chicken retina after laser photocoagulation
Author(s) -
Takeyama Masayuki,
Yoneda Masahiko,
Takeuchi Makoto,
Isogai Zenzo,
OhnoJinno Akiko,
Kataoka Takuya,
Li Huili,
Sugita Iichiro,
Iwaki Masayoshi,
Zako Masahiro
Publication year - 2010
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20931
Subject(s) - retina , zymography , inner limiting membrane , matrix metalloproteinase , retinal , ophthalmology , in situ hybridization , chemistry , pathology , anatomy , microbiology and biotechnology , biology , messenger rna , medicine , biochemistry , neuroscience , gene
Abstract Background and Objective We investigated the levels of matrix metalloproteinase‐2 (MMP‐2), which has been implicated in various vitreoretinal diseases, in the retina after laser photocoagulation (LPC). Materials and Methods The time course of MMP‐2 expression in 2‐day‐old chicken retinas before and 6 hours, 12 hours, 1 day, 2 days, 4 days, 8 days, 16 days, and 32 days after LPC was determined by real‐time PCR and gelatin zymography. The basal level of MMP‐2 in the retina and vitreous was also measured by gelatin zymography. MMP‐2 localization in the retina was examined by immunohistochemistry. The localization of MMP‐2 mRNA was determined by fluorescent in situ hybridization. The internal limiting membrane (ILM) was observed by scanning electron microscopy. Results MMP‐2 mRNA expression in the retina peaked at day 4, but gelatin zymography showed that MMP‐2 peaked 6 hours after LPC and the significant increase in the level of active MMP‐2 lasted for more than 4 days. The concentration of MMP‐2 in the vitreous was significantly higher than that in the retina. A distinct MMP‐2 signal around the ILM was identified 6 hours after LPC, but MMP‐2 mRNA was not detected there. Electron microscopy showed a damaged retinal surface after LPC. Conclusion and Outlook The significant increase in retinal MMP‐2 which lasted for more than 4 days after LPC may be induced by influx from the vitreous into the retina. This MMP‐2 dynamics may contribute to pathological processes in the retina after LPC. Lasers Surg. Med. 42:433‐441, 2010. © 2010 Wiley‐Liss, Inc.