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Biochemical and morphological analysis of dentin following selective caries removal with a fluorescence‐controlled Er:YAG laser
Author(s) -
Jepsen Søren,
Açil Yahya,
Peschel Thorsten,
Kargas Konstantinos,
Eberhard Jörg
Publication year - 2008
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20631
Subject(s) - dentin , fluorescence , er:yag laser , dentistry , chemistry , laser , confocal laser scanning microscope , materials science , biomedical engineering , medicine , optics , physics
Background and Objective We evaluated the endpoint of caries removal with a novel fluorescence‐controlled Er:YAG laser by biochemical analysis of dentin collagen and electron microscopy. Materials and Methods For the biochemical analysis 210 carious and 60 caries‐free teeth were treated by a fluoresence controlled Er:YAG laser ( n = 240) or by conventional drilling ( n = 30). A layer of dentin from the cavity floor was removed to determine collagen crosslinks hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) by HPLC. Thirty teeth were examined by SEM and TEM. Results Approximately 100% HP and LP were found in carious dentin, in contrast to 0.33% (HP) and 0.68% (LP) in sound dentin. Following caries removal by laser 0.84% (HP) and 1.26% (LP) and 1.56% (HP) and 2.48% (LP) (threshold level 5, respectively 10) were found. Conclusion The biochemical and ultrastructural analysis demonstrated the suitability of the fluorescence controlled Er:YAG laser to remove irreversibly denatured collagen of carious dentin. Lesers Surg. Med. 40:350–357, 2008. © 2008 Wiley‐Liss, Inc.