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Laser treatments on skin enhancing and controlling transdermal delivery of 5‐fluorouracil
Author(s) -
Gómez Clara,
Costela Ángel,
GarcíaMoreno Inmaculada,
Llanes Felipe,
Teijón José M.,
Blanco Dolores
Publication year - 2008
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20590
Subject(s) - stratum corneum , transdermal , irradiation , laser , materials science , ablation , ultraviolet , penetration (warfare) , human skin , biomedical engineering , optics , optoelectronics , pathology , medicine , physics , operations research , biology , nuclear physics , pharmacology , genetics , engineering
Background and Objective Laser ablation of stratum corneum (SC) enhances transdermal delivery of hydrophilic drugs. The influence of the infrared (IR) ( λ = 1,064 nm), visible ( λ = 532 nm), and ultraviolet (UV) ( λ = 355 nm) radiations of a Nd:YAG laser on transdermal delivery of 5‐Fluorouracil (5‐Fu) across skin was studied in vitro. Materials and Methods Pinna skin of the inner side of rabbit ear, was used for the skin permeation. The light source for laser treatment was a Q‐switched Nd:YAG laser (Lotis TII SL‐2132). Ablation thresholds were estimated by using a photoacoustic technique. In addition, permeation study, and morphological and structural skin examination by histology and differential scanning calorimetry (DSC) were carried out. Results A significant increase in the permeation of 5‐Fu across skin pre‐treatment with the three different wavelengths studied was obtained. Since irradiation at 1,064 nm allows deep penetration of the radiation, collagen fibers were affected [7.7 J/cm 2 (15 Hz)]. Visible radiation of Nd:YAG laser showed the wider range of fluences (3–8.4 J/cm 2 at 15 Hz) to enhance skin delivery of 5‐Fu, without risk of skin lesion. UV radiation required minor energy contribution to produce the same effects within a narrower range of fluences [0.3 J/cm 2 (5 Hz)–1.5 J/cm 2 (15 Hz)] so the process is less controlled and this radiation shows greater impact on the lipidic structure than visible and IR radiations. Conclusions Use of the visible radiation of a Nd:YAG laser is a good method for improving the efficacy of topical chemotherapy of 5‐Fu. Lesers Surg. Med. 40:6–12, 2008. © 2008 Wiley‐Liss, Inc.