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Increased expression of mitochondrial benzodiazepine receptors following low‐level light treatment facilitates enhanced protoporphyrin IX production in glioma‐derived cells in vitro
Author(s) -
Bisland S.K.,
Goebel E.A.,
Hassanali N.S.,
Johnson C.,
Wilson B.C.
Publication year - 2007
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20544
Subject(s) - photosensitizer , protoporphyrin ix , photodynamic therapy , viability assay , glioma , chemistry , in vitro , receptor , cell , western blot , microbiology and biotechnology , pharmacology , biophysics , cancer research , biochemistry , biology , photochemistry , gene , organic chemistry
Background and objectives This study investigates whether low‐level light treatment (LLLT) can enhance the expression of peripheral‐type mitochondrial benzodiazepine receptors (PBRs) on glioma‐derived tumor cells, and by doing so promote the synthesis of protoporphyrin IX (PpIX) and increase the photodynamic therapy (PDT)‐induced cell kill using 5‐aminolevulinic acid (ALA). The endogenous photosensitizer, PpIX and related metabolites including coproporphyrin III are known to traffic into or out of the mitochondria via the PBRs situated on the outer mitochondrial membrane. Cells of astrocytic derivation within the brain express PBRs, while neurons express the central‐type of benzodiazepine receptor. Study Design Astrocytoma‐derived CNS‐1 cells were exposed to a range of differing low‐level light protocols immediately prior to PDT. LLLT involved using broad‐spectrum red light of 600–800 nm or monochromatic laser light specific to 635 or 905 nm wavelength. Cells (5×10 5 ) were exposed to a range of LLLT doses (0, 1, or 5 J/cm 2 ) using a fixed intensity of 10 mW/cm 2 and subsequently harvested for cell viability, immunofluorescence, or Western blot analysis of PBR expression. The amount of PpIX within the cells was determined using chemical extraction techniques. Results Results confirm the induction of PBR following LLLT is dependent on the dose and wavelength of light used. Broad‐spectrum red light provided the greatest cell kill following PDT, although LLLT with 635 nm or 905 nm also increased cell kill as compared to PDT alone. All LLLT regimens increased PBR expression compared to controls with corresponding increases in PpIX production. Conclusions These data suggest that by selectively increasing PBR expression in tumor cells, LLLT facilitates enhanced tumor cell kill using ALA‐PDT. This may further improve the selectivity and efficacy of PDT treatment of brain tumors. Lasers Surg. Med. 39:678–684, 2007. © 2007 Wiley‐Liss, Inc.