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Effectiveness of narrow‐band ultraviolet‐B phototherapy for prevention of intimal hyperplasia in a rat carotid balloon injury model
Author(s) -
Kohyama Shinya,
Morimoto Yuji,
Nakai Kanji,
Kaji Tatsumi,
Tokumaru Aya,
Nawashiro Hiroshi,
Shima Katsuji,
Satoh Yasushi,
Takishima Kunio,
Kikuchi Makoto,
Matsuo Hirotaka
Publication year - 2007
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20543
Subject(s) - intimal hyperplasia , hyperplasia , flow cytometry , pathology , medicine , cell , chemistry , cancer research , smooth muscle , immunology , biochemistry
Background and Objective Narrow‐band ultraviolet‐B light (NBUVB) (313 nm) is known to have anti‐proliferative effects, implying a potential treatment for intimal hyperplasia, but it remains to be ascertained. We assessed the effects of NBUVB irradiation for prevention of intimal hyperplasia. Study Design/Materials and Methods The rat carotid arteries were irradiated with NBUVB after balloon injury (BI), and the degree of intimal hyperplasia was histopathologically assessed. The anti‐proliferative effects using cultured human smooth muscle cells were evaluated by flow cytometry and immunoblot analysis. Results NBUVB (0.3–4.5 J/cm 2 ) irradiation immediately after BI reduced the degree of intimal hyperplasia at 14 and 28 days after BI ( P <0.001) without any obvious complications. Neither an increase in the number of medial cells nor upregulation of proliferating cell nuclear antigen was observed in the irradiated arteries. NBUVB irradiation at 2 or 14 days after BI significantly suppressed further intimal hyperplasia ( P <0.01). NBUVB‐irradiated cultured cells showed inhibited proliferation involved with G 1 and G 2 /M arrests. Increased expression of p53 and inhibition of retinoblastoma protein (pRB) phosphorylation were also seen in the NBUVB‐irradiated cells. Conclusions These data suggest that NBUVB irradiation is an effective method for preventing intimal hyperplasia. The anti‐proliferative effect is partly due to the cell cycle arrest caused by p53 expression and inhibited pRB phosphorylation. Lasers Surg. Med. 39:659–666, 2007. © 2007 Wiley‐Liss, Inc.