Premium
Multiphoton microscopy for monitoring intratissue femtosecond laser surgery effects
Author(s) -
Wang BaoGui,
Riemann Iris,
Schubert Harald,
Schweitzer Dietrich,
König Karsten,
Halbhuber KarlJuergen
Publication year - 2007
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20523
Subject(s) - femtosecond , microscopy , laser , materials science , tomography , biomedical engineering , in vivo , microscope , optics , two photon excitation microscopy , laser surgery , medicine , pathology , biology , microbiology and biotechnology , fluorescence , physics
Background and Objective Multiphoton microscopy/tomography has been used as a novel diagnostic method for corneal imaging with subcellular resolution. Here, we used this technique to monitor femtosecond laser intratissue surgery effects. Materials and Methods Multiphoton microscopy/tomography on rabbits based on intense 90 MHz femtosecond Ti: sapphire laser was realized at intensities of MW‐GW/cm 2 , whereas the surgical procedures were performed with the same system at a higher light intensity on the order of TW/cm 2 . Results Multiphoton microscopy/tomography proved capable of determining the target of interest, and of visualizing and optically evaluating the in vivo intrastromal laser surgical outcomes with high efficiency. More interesting, using this technique, activated intrastromal keratocytes (myofibroblasts) were detected in vivo 24 hours after intrastromal surgery. Conclusion Multiphoton microscopy/tomography is an efficient and convenient non‐invasive imaging method which can be used not only to assess intrastromal microsurgical performance but also to perform in vivo follow‐up observations on wound repair. Lasers Surg. Med. 39:527–533, 2007. © 2007 Wiley‐Liss, Inc.