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Microtomographic analysis of healing of femtosecond laser bone calvarial wounds compared to mechanical instruments in mice with and without application of BMP‐7
Author(s) -
Girard B.,
Cloutier M.,
Wilson D.J.,
Clokie C.M.L.,
Miller R.J.D.,
Wilson B.C.
Publication year - 2007
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20493
Subject(s) - calvaria , femtosecond , wound healing , laser , biomedical engineering , bone healing , medicine , dentistry , surgery , materials science , chemistry , optics , biochemistry , physics , in vitro
Background and Objective This study investigated the healing of femtosecond laser created wounds in an animal model. Study Design We have assessed the healing of critical size wounds in mice calvaria using three different wounding techniques: carbide bur, diamond end‐cutting bur, and ultrafast femtosecond laser, and in the presence or absence of bone morphogenetic protein‐7 (BMP). Wound closure was examined using microcomputerized tomography at 3, 6, 9, and 12 weeks. Results Results have shown partial closure at up to 12 weeks with all techniques that did not involve the use of BMP, with the least closure noted in the laser groups as suggested by two‐dimensional radiographic analysis. Bone volume measurements appeared slightly lower for the laser than for the mechanical groups, however statistically significant differences were seen only at week 6. No significant differences in closure were noted for the different methods in the BMP treated groups. Conclusions Femtosecond laser cutting demonstrated an unsurpassed precision when compared to mechanical instruments. The addition of BMP led to very rapid healing with complete closure seen as early as 3 weeks and overcomes any potential healing delays that may arise from laser tissue cutting. Lasers Surg. Med. 39:458–467, 2007. © 2007 Wiley‐Liss, Inc.