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Correlation of photodynamic activity and fluorescence signaling for free and pegylated mTHPC in mesothelioma xenografts
Author(s) -
Tran Nam,
Krueger Thorsten,
Pan Youmin,
Yan Hua,
Cheng Cai,
Altermatt HansJörg,
Ballini JeanPierre,
Borle François,
Ris HansBeat,
AndrejevicBlant Snezana
Publication year - 2007
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20477
Subject(s) - photodynamic therapy , phototoxicity , chemistry , pegylation , cancer research , pathology , medicine , in vitro , biochemistry , polyethylene glycol , organic chemistry
Background/Objectives Correlation of photodynamic activity (PDT) and fluorescence signaling for free and pegylated meta‐tetrahydroxyphenylchlorin (mTHPC) in nude mice with mesothelioma xenografts. Study Design/Materials and Methods Twelve animals received light delivery (20 J/cm 2 , 150 mW/cm 2 , spot size 1.2 cm) on the tumor and the hind leg 3 days after sensitization with 0.15 mg/kg free mTHPC ( n = 6) or equimolar‐dosed pegylated mTHPC ( n = 6). Groups of three animals each were sensitized with 0.15 and 0.5 mg/kg free mTHPC or equimolar dosed pegylated mTHPC followed after 3 days by fluorescence microscopy measurements. Results Pegylated mTHPC resulted in a similar extent of PDT‐related tumor necrosis but in lower skin phototoxicity than free mTHPC. Both mTHPC formulations were heterogeneously distributed in the tumor and were mainly localized in perivascular areas. Pegylated mTHPC revealed a higher tumor to skin fluorescence intensity ratio than free mTHPC ( P <0.001). Conclusions Fluorescence signaling measurement has the potential to predict the photodynamic activity for both mTHPC formulations in mesothelioma xenografts. Lasers Surg. Med. 39:237–244, 2007. © 2007 Wiley‐Liss, Inc.