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Effect of optical clearing agents on the in vivo optical properties of squamous epithelial tissue
Author(s) -
Millon Stacy R.,
RoldanPerez Katherine M.,
Riching Kristin M.,
Palmer Gregory M.,
Ramanujam Nirmala
Publication year - 2006
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20451
Subject(s) - cheek pouch , attenuation coefficient , in vivo , dimethyl sulfoxide , materials science , absorption (acoustics) , scattering , ex vivo , penetration depth , penetration (warfare) , chemistry , wavelength , hamster , biophysics , optics , biomedical engineering , optoelectronics , medicine , composite material , biology , physics , microbiology and biotechnology , organic chemistry , operations research , engineering
Background and Objectives Optical clearing agents (OCAs) have previously been shown to increase depth penetration within turbid tissue ex vivo. This paper quantifies tissue optical properties of the hamster cheek pouch model in order to provide a means to assess the effect of OCAs quantitatively in vivo. Study Design/Materials and Methods Diffuse reflectance spectra were obtained from both cheeks of 12 hamsters before and after immersion in dimethyl sulfoxide (DMSO), glycerol or a phosphate buffer saline (PBS) control for 20 minutes. A Monte Carlo model was then utilized to derive the wavelength dependent reduced scattering and absorption coefficients. Results DMSO caused a statistically significant decrease in the absorption and reduced scattering coefficients derived by the model. Glycerol caused a statistically significant increase in the wavelength dependent absorption coefficient, but no statistically significant changes in the reduced scattering coefficient. Conclusions DMSO and glycerol act upon tissues differently as reflected by the tissue optical properties, implying that not all OCAs are equally effective in optically clearing tissues. Lasers Surg. Med. 38:920–927, 2006. © 2006 Wiley‐Liss, Inc.