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Effects of Argon laser irradiation on polar excitations in frog sciatic nerve
Author(s) -
Matsuda Yoshiki,
Niwa Mayuka,
Iwai Hiromasa,
Kogure Shinichi,
Honjoe Nobuyuki,
Komatsu Mitsuaki,
Ishii Yoshio,
Watanabe Kazuhiro
Publication year - 2006
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20343
Subject(s) - irradiation , laser , xenopus , chemistry , sciatic nerve , excitation , cathode , hyperpolarization (physics) , biophysics , materials science , optics , anatomy , biology , biochemistry , physics , electrical engineering , organic chemistry , nuclear magnetic resonance spectroscopy , nuclear physics , gene , engineering
Background and Objectives Since the mechanisms underlying the effects of low‐power laser irradiation on the nervous system remain unclear, we examined whether such irradiation can influence ionic channels of the nerve membrane using the law of polar excitation in isolated frog sciatic nerve. Study Design/Materials and Methods Using 43 frogs ( Xenopus laevis ), nerve preparations were stimulated at 0.5/second using a 10‐millisecond pulse at supramaximal intensity. Ar + laser irradiation (457, 488, 514 nm; 50, 75, 100 mW) was applied for 30 minutes to the portion between the anode and cathode stimulating electrodes. Results and Conclusions Ar + laser irradiations (457, 488 nm; 50 mW) blocked the generation of anode‐break‐excitation, rather than cathode‐make‐excitation. Such a selective effect occurred when applying a blocker of hyperpolarization‐activated cation current (Ih) channel, ZD7288. Ar + laser irradiation may influence Na + channels in addition to Ih channels. Lasers Surg. Med. 38:608–614, 2006. © 2006 Wiley‐Liss, Inc.