Premium
Autofluorescence properties of rat cerebellum cortex during postnatal development
Author(s) -
Croce Anna C.,
Pisu Maria B.,
Roda Elisa,
Avella Debora,
Bernocchi Graziella,
Bottiroli Giovanni
Publication year - 2006
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20336
Subject(s) - autofluorescence , histogenesis , in vivo , cerebellum , pathology , chemistry , anatomy , cortex (anatomy) , ex vivo , biophysics , fluorescence , biology , medicine , neuroscience , immunohistochemistry , optics , physics , microbiology and biotechnology
Background and Objectives The multilayered structure of rat neocerebellum cortex (VI–VIII lobules of the vermis) during postnatal development undergoes rearrangements, which in turn are affected by treatment with the anti‐tumoral drug cisplatin. The dependence of autofluorescence emission properties on the tissue structural and molecular features has been investigated. Study design/Materials and Methods Autofluorescence analysis was performed at defined time points of cerebellar histogenesis—11, 17, and 30 postnatal days‐ under normal conditions or after 5 µg/g body weight cisplatin treatment at 10 postnatal day. Autofluorescence signal was analyzed in vivo at the surface of intact lobules of cerebellum vermis by means of fiber optic spectrofluorometry, or on tissue sections by means of microspectrofluorometry and fluorescence imaging. Results In vivo spectroscopy showed changes of autofluorescence signal both during normal histogenesis and after cisplatin treatment. External granular layer (EGL) and molecular layer (ML), that is, the more external layers were found to be interested by structural alterations, and showed the greatest changes in signal amplitude, accounting for the in vivo results. Fitting analysis indicated that changes in spectral shape reflected an increase in oxidative damages induced by cisplatin treatment. Conclusions The results confirm the relationship of the autofluorescence emission properties with histological and biochemical features of biological tissue. Lasers Surg. Med. 38:598–607, 2006. © 2006 Wiley‐Liss, Inc.