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Comparing and combining light dose fractionation and iron chelation to enhance experimental photodynamic therapy with aminolevulinic acid
Author(s) -
Curnow Alison,
MacRobert Alexander J.,
Bown Stephen G.
Publication year - 2006
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20328
Subject(s) - chelation , fractionation , photodynamic therapy , chemistry , chelation therapy , chromatography , inorganic chemistry , organic chemistry
Abstract Background and Objectives Enhancement of photodynamic therapy (PDT) with 5‐aminolevulinic acid (ALA) has been demonstrated experimentally using light dose fractionation or CP94 iron chelation. This study extends this research. Study Design/Materials and Methods In normal rat colon, CP94 administration and light dose fractionation were independently and concurrently employed to enhance ALA‐PDT. In colonic rat tumors, the most successful enhancement regimes were employed separately. Results Independent use of light dose fractionation and iron chelation produced similar results in normal colon (2.4‐ and 2.9‐fold more necrosis than controls, respectively). Using both techniques simultaneously produced fivefold enhancement. In the colonic tumors, light dose fractionation and iron chelation (using different parameters) produced two and five times the volume of necrosis, respectively. Conclusions Both techniques significantly enhanced ALA‐PDT in the normal and neoplastic tissues investigated and produced similar levels of enhancement when comparable parameters were employed. Concurrent use of light dose fractionation and iron chelation in normal colon produced considerably more enhancement than either technique could achieve independently. Lasers Surg. Med. 38:325–331, 2006. © 2006 Wiley‐Liss, Inc.