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Cell nanosurgery using ultrashort (femtosecond) laser pulses: Applications to membrane surgery and cell isolation
Author(s) -
Kohli Vikram,
Elezzabi Abdulhakem Y.,
Acker Jason P.
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20220
Subject(s) - femtosecond , ablation , laser , laser surgery , materials science , membrane , optics , chemistry , medicine , physics , biochemistry
Membrane surgery and nanosurgical cell isolation using high‐intensity femtosecond laser pulses is reported. We demonstrate the applicability of using ultrashort (femtosecond) laser pulses for performing surgery on live mammalian cells. When sub‐10 femtosecond pulses were focused onto the cell, precise sub‐micron surgical cuts were made on the biological membrane. Traversing the cells relative to the focused laser spot, we report on localized nanosurgical ablation of focal adhesion adjoining epithelial cells. In each study, the surgery was conducted without morphologically compromising the cells. © 2005 Wiley‐Liss, Inc.