Premium
Low power laser irradiation alters gene expression of olfactory ensheathing cells in vitro
Author(s) -
Byrnes Kimberly R.,
Wu Xingjia,
Waynant Ronald W.,
Ilev Ilko K.,
Anders Juanita J.
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20202
Subject(s) - ciliary neurotrophic factor , neurotrophic factors , olfactory ensheathing glia , neurite , transplantation , glial cell line derived neurotrophic factor , downregulation and upregulation , immunolabeling , brain derived neurotrophic factor , in vitro , microbiology and biotechnology , chemistry , biology , pathology , medicine , olfactory bulb , neuroscience , central nervous system , immunohistochemistry , biochemistry , receptor , gene
Background and Objectives Both photobiomodulation (PBM) and olfactory ensheathing cells (OECs) transplantation improve recovery following spinal cord injury. However, neither the combination of these two therapies nor the effect of light on OECs has been reported. The purpose of this study was to determine the effect of light on OEC activity in vitro. Materials and Methods OECs were purified from adult rat olfactory bulbs and exposed to 810 nm light (150 mW; 0, 0.2, or 68 J/cm 2 ). After 7–21 days in vitro, cells underwent immunocytochemistry or RNA extraction and RT‐PCR. Results Analysis of immunolabeling revealed a significant decrease in fibronectin expression in the cultures receiving 68 J/cm 2 . Analysis of gene expression revealed a significant ( P < 0.05) increase in brain derived neurotrophic factor (BDNF), glial derived neurotrophic factor (GDNF), and collagen expression in the 0.2 J/cm 2 group in comparison to the non‐irradiated and 68 J/cm 2 groups. OEC proliferation was also found to significantly increase in both light treated groups in comparison to the control group ( P < 0.001). Conclusions These results demonstrate that low and high dosages of PBM alter OEC activity, including upregulation of a number of neurotrophic growth factors and extracellular matrix proteins known to support neurite outgrowth. Therefore, the application of PBM in conjunction with OEC transplantation warrants consideration as a potential combination therapy for spinal cord injury. Lasers Surg. Med. © 2005 Wiley‐Liss, Inc.