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Helium–neon laser in viability of random skin flap in rats
Author(s) -
Pinfildi Carlos E.,
Liebano Richard E.,
Hochman Bernardo S.,
Ferreira Lydia M.
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20190
Subject(s) - irradiation , laser , skin flap , surgery , medicine , nuclear medicine , optics , physics , nuclear physics
Abstract Background and Objectives The purpose of this study was to determine the role of helium–neon (He–Ne) laser random skin flap viability in rats. Study Design/Materials and Methods Experimentally controlled randomized study.Forty‐eight Wistar‐EPM rats were used, weighed, and divided into 4 groups with 12 rats each. The random skin flap was performed measuring 10×4 cm, with a plastic sheet interposed between the flap and the donor site. The Group 1 (control) underwent sham irradiation with He–Ne laser. The Group 2 was submitted to laser irradiation, using the punctual contact technique on the skin flap surface. The Group 3 was submitted to laser irradiation surrounding the skin flap, and the Group 4 was submitted to laser irradiation both on the skin flap surface and around it. The experimental groups were submitted to He–Ne laser irradiation with 3 J/cm 2 energy density immediately after the surgery and for the four subsequent days. The percentage of necrotic area of the four groups was calculated at the 7th post‐operative day, through a paper‐template method. Results Group 1 reached an average necrotic area of 48.86%; Group 2, 38.67%; Group 3, 35.34%; and Group 4, 22.61%. After the statistic analysis, results showed that all experimental groups reached statistically significant values when compared to the control group, and Group 4 was the best one, when compared to all groups of this study ( P <0.001). Conclusion The He–Ne laser irradiation was efficient to increase random skin flap viability in rats. © 2005 Wiley‐Liss, Inc.