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Effect of the 1,450 nm diode non‐ablative laser on collagen expression in an artificial skin model
Author(s) -
Nouri Keyvan,
Zhang YanPing,
Singer Lauren,
Zhu Linjian,
Huo Ran,
Ricotti Carlos,
Prodanovich Srdjan,
Li Jie
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20189
Subject(s) - elastin , laser , fluence , biomedical engineering , materials science , diode , messenger rna , protein expression , ablative case , optics , chemistry , pathology , medicine , optoelectronics , surgery , gene , physics , radiation therapy , biochemistry
Background and Objective The 1,450‐nm Smoothbeam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non‐ablative laser on Apligraf® (bioengineered skin‐substitute) and to document its use as a model for non‐ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10 J, 12J, and 14J. The sheets were then incubated with 10% CO 2 at 37°C and samples were collected and analyzed 3 days later, using RT‐PCR and immunofluorescent staining. Results Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12 J. Conclusions There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J. © 2005 Wiley‐Liss, Inc.