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Qualitative microanalysis of ions and ultrastructural changes in dentin exposed to laser irradiation and to metal salts solution
Author(s) -
Glauche Carlos E.,
de Freitas Patricia M.,
Vieira Nilson D.,
Lage Marques José Luiz
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20153
Subject(s) - dentin , dentinal tubule , microanalysis , scanning electron microscope , irradiation , nuclear chemistry , chemistry , metal , aqueous solution , ultrastructure , laser , dentistry , materials science , anatomy , composite material , medicine , physics , organic chemistry , nuclear physics , optics
Background and Objectives This study evaluated the ultrastructural changes in dentin after treatment with the Nd:YAG laser and/or metal salt solutions and verified the presence of Sn ++ , Sr ++ , and F − in dentin structure. Study Designs/Materials and Methods Sixty dentin disks were randomly divided into groups (n = 10): (I) control (no treatment), (II) Nd:YAG (1.5 W, 100 mJ, 15 Hz, 125 J/cm 2 ), (III) 10% SnF 2 aqueous solution for 30 minutes, (IV) Nd:YAG+10% SnF 2 aqueous solution for 30 minutes, (V) 10% SrCl 2 toothpaste for 30 minutes, (VI) Nd:YAG+10% SrCl 2 toothpaste for 30 minutes. Then, all samples were prepared for scanning electron microscopy (SEM) and the samples from Groups I to IV for the energy dispersive X‐ray microanalysis (EDX). Results SEM evaluation revealed occluded dentinal tubules and a dentin surface altered by the laser irradiation. The EDX microanalysis revealed Sn ++ at a depth of 250 μm in Group IV and not deeper than 100 μm in Group III. In Group V, Sr ++ was not deeper than 50 μm, but it could be detected at a depth of 500 μm in Group VI. F − was found only in Group IV. Conclusions Ultrastructural changes caused by laser irradiation can increase dentin uptake of Sn ++ , Sr ++ , and F − . © 2005 Wiley‐Liss, Inc.