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Cellular effects of low power laser therapy can be mediated by nitric oxide
Author(s) -
Karu Tiina I.,
Pyatibrat Ludmila V.,
Afanasyeva Natalia I.
Publication year - 2005
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/lsm.20148
Subject(s) - nitric oxide , sodium nitroprusside , irradiation , biophysics , chemistry , action spectrum , ascorbic acid , photochemistry , organic chemistry , physics , food science , nuclear physics , biology
Background and Objectives The objective of this study was to investigate the possibility of involvement of nitric oxide (NO) into the irradiation‐induced increase of cell attachment. These experiments were performed with a view to exploring the cellular mechanisms of low‐power laser therapy. Study Design/Materials and Methods A suspension of HeLa cells was irradiated with a monochromatic visible‐to‐near infrared radiation (600–860 nm, 52 J/m 2 ) or with a diode laser (820 nm, 8–120 J/m 2 ) and the number of cells attached to a glass matrix was counted after 30 minute incubation at 37°C. The NO donors sodium nitroprusside (SNP), glyceryl trinitrate (GTN), or sodium nitrite (NaNO 2 ) in the concentration range 5 × 10 −9 –5 × 10 −4 M were added to the cellular suspension before or after irradiation. The action spectra and the concentration and fluence dependencies obtained were compared and analyzed. Results The well‐structured action spectrum for the increase of the adhesion of the cells, with maxima at 619, 657, 675, 740, 760, and 820 nm, points to the existence of a photoacceptor responsible for the enhancement of this property (supposedly cytochrome c oxidase, the terminal respiratory chain enzyme), as well as signaling pathways between the cell mitochondria, plasma membrane, and nucleus. Treating the cellular suspension with SNP (5 × 10 −5 M) before irradiation significantly modifies the action spectrum for the enhancement of the cell attachment property (band maxima at 642, 685, 700, 742, 842, and 856 nm). The action of SNP, GTN, and NaNO 2 added before or after irradiation depends on their concentration and radiation fluence. Conclusions The NO donors added to the cellular suspension before irradiation eliminate the radiation‐induced increase in the number of cells attached to the glass matrix, supposedly by way of binding NO to cytochrome c oxidase. NO added to the suspension after irradiation can also inhibit the light‐induced signal downstream. Both effects of NO depend on the concentration of the NO donors added. These results indicate that NO can control the irradiation‐activated reactions that increase the attachment of cells. © 2005 Wiley‐Liss, Inc.